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Item #:
35091069
50X TAE Buffer (Tris-Acetate-EDTA), 500 mL, #10417 from Cepham Life Sciences
Item #:
35091069
SCR 1290
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Cepham Life Sciences 50X TAE Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH.
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What Stands Out
Optimal Buffering
This 50X TAE Buffer maintains stable pH levels during electrophoresis, ensuring consistent results for nucleic acid separation, crucial for laboratories conducting DNA and RNA analysis.
High Purity
Formulated with ultra-pure Tris, acetate, and EDTA, minimizing contaminants that could interfere with experiments. This ensures accuracy and reliability in sensitive biochemical assays.
Convenient Volume
Packaged in a 500 mL bottle, it's designed for easy handling and storage, making it ideal for frequent lab use while reducing waste and enhancing cost-effectiveness for researchers.
Product Details
- Cepham Life Sciences 50X TAE Buffer contains 2M Tris, 1M Acetic acid with 0.05M EDTA
- Used for running electrophoresis of nucleic acids in gels
- Provides electrical conductivity and maintains pH
- EDTA inhibits metal dependent nucleases, protecting DNA during electrophoresis
- Suitable for genomic and large supercoiled DNA, and as a gel preparation buffer
- 50X concentrated TAE buffer should be diluted with molecular biology grade water to 1X before use
| % Assay of Chemical(s) | 50 |
| Container Type | Bottle |
| Item Form | Gel |
| Volume | 500 ml |
| Brand Name | Cepham Life Sciences |
| Manufacturer | Cepham Life Sciences |
| Part Number | 10417 |
| Brand | Cepham Life Sciences |
| Item Volume | 500 ml |
| Percentage assay | 50 |
| Package Information | Bottle |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists conducting DNA electrophoresis and requiring reliable TAE buffer for optimal performance.
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Educational Institutions
Perfect for colleges and universities in teaching labs where DNA analysis and electrophoresis are part of the curriculum.
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Research Laboratories
Suitable for research labs focusing on genetic studies, needing high-quality buffer solutions for detailed experiments.
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Basic Users
Not ideal for casual or basic users who lack expertise in handling specialized reagents like TAE buffer.
Product Description
About This Item
Introducing the Cepham Life Sciences 50X TAE Buffer Tris-Acetate-EDTA, 500 mL! Are you looking for a reliable and efficient buffer solution for your nucleic acid electrophoresis experiments? Look no further than our top-quality TAE buffer. Cepham Life Sciences 50X TAE Buffer is specially formulated to provide the perfect electrical conductivity and pH maintenance for running electrophoresis of nucleic acids in gels. With a composition of 2M Tris, 1M Acetic acid, and 0.05M EDTA, this buffer ensures optimal performance in your experiments. One of the key features of our TAE buffer is the presence of EDTA, which acts as a potent inhibitor of metal-dependent nucleases. By chelating divalent cations like Ca2+ and Mg2+, EDTA protects your precious DNA from degradation during electrophoresis, ensuring accurate and reliable results. Versatility is another standout feature of our TAE buffer.
It is suitable for both genomic and large supercoiled DNA, making it a versatile choice for your research needs. Additionally, it can also be used as a gel preparation buffer, further enhancing its convenience and value. It's important to note that the buffering capacity of TAE buffer is slightly lower than TBE buffer. As such, we recommend periodic replacement of the buffer during extended electrophoresis.
However, TAE buffer offers the advantage of faster dsDNA runs compared to TBE buffer. To ensure optimal performance, the 50X concentrated TAE buffer should be diluted with molecular biology grade water to a 1X concentration before running your electrophoresis experiments. With our commitment to quality and excellence, Cepham Life Sciences offers this high-grade TAE Buffer in various sizes, including 500 mL, 1 L, and 1 Gal. Choose the size that best suits your laboratory's needs. Please note that our TAE Buffer is for research use only and not intended for human or animal clinical/therapeutic use. For your safety, please follow all safety instructions provided. When it comes to purchasing the Cepham Life Sciences 50X TAE Buffer Tris-Acetate-EDTA, look no further than Ubuy.
We are your trusted source for all your laboratory needs. Don't miss out on the opportunity to elevate your research to new heights. Place your order today!.
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Customer Questions & Answers
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Question:
What is 50X TAE Buffer Tris-Acetate-EDTA used for?
Answer: 50X TAE Buffer Tris-Acetate-EDTA is primarily used in molecular biology for techniques such as gel electrophoresis. It facilitates the separation of nucleic acids, such as DNA and RNA, through an electric field. The buffer maintains a stable pH during the electrophoresis process, protecting the integrity of samples. This makes it essential for tasks like DNA fragment analysis, cloning, and sequencing. -
Question:
How do you prepare 1X TAE Buffer from 50X TAE Buffer?
Answer: To prepare a 1X TAE Buffer from the 50X stock, simply dilute the 50X solution. Mix 10 mL of the 50X TAE Buffer with 490 mL of distilled water to achieve a total volume of 500 mL. This diluted buffer can directly be used for applications such as gel preparation and running electrophoresis, ensuring optimal sample resolution. -
Question:
What is the shelf life of 50X TAE Buffer?
Answer: The shelf life of 50X TAE Buffer can typically be extensive when stored under appropriate conditions. It should be kept at room temperature and protected from direct light and contaminants. When stored properly, it can remain stable for a prolonged period, making it a reliable choice for laboratories requiring consistent performance in various applications. -
Question:
Is 50X TAE Buffer suitable for use with agarose gels?
Answer: Yes, 50X TAE Buffer is suitable for use with agarose gels. It provides optimal conductivity and pH that aids in the effective separation of nucleic acids during electrophoresis. This buffer helps to maintain the stability of DNA or RNA samples, allowing for better resolution and clearer results when visualized post-electrophoresis. -
Question:
Can 50X TAE Buffer be used for protein electrophoresis?
Answer: No, 50X TAE Buffer is not suitable for protein electrophoresis, as it is specifically formulated for nucleic acids. For protein separation, buffers like SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) should be used. This distinction is important as the composition of the buffer can significantly impact the separation quality of different biomolecules. -
Question:
What are the safety precautions when handling 50X TAE Buffer?
Answer: When handling 50X TAE Buffer, it is recommended to wear gloves and safety goggles to avoid any contact with skin or eyes. Although it is generally safe, always follow laboratory safety guidelines. Proper lab attire and working in a well-ventilated area ensure a safe handling environment, which is crucial for maintaining personal safety during experiments. -
Question:
How does TAE Buffer differ from TBE Buffer?
Answer: TAE Buffer (Tris-Acetate-EDTA) differs from TBE Buffer (Tris-Borate-EDTA) in composition and application. TAE is primarily used for DNA or RNA electrophoresis, allowing for better separation of larger fragments, whereas TBE is preferred for higher-resolution applications, such as smaller fragments. The choice between TAE and TBE buffers can affect the results based on the experimental requirements. -
Question:
What is the significance of pH in TAE Buffer?
Answer: The pH level of TAE Buffer is crucial as it influences the stability of nucleic acids during electrophoresis. A stable pH helps maintain the charge and structure of DNA or RNA, which is essential for effective separation and analysis. Consistent pH levels can enhance the reproducibility of experiments, making TAE Buffer a reliable choice for molecular biology applications. -
Question:
How can I store 50X TAE Buffer for long-term use?
Answer: For long-term storage of 50X TAE Buffer, keep it in a tightly sealed container and store it away from light and extreme temperatures. Ideally, it should be stored at room temperature in a cool, dark place. Proper storage extends its usability, ensuring that it remains effective for various laboratory applications whenever needed. -
Question:
Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 500 mL from Cepham Life Sciences?
Answer: You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 500 mL, 10417 from Ubuy in Seychelles. Ubuy offers a reliable platform for obtaining laboratory supplies, ensuring you get quality products for your research needs.
Cepham Life Sciences Buffers Editorial Review
The 50X TAE buffer from Cepham Life Sciences is a reliable and high-quality solution for molecular biology experiments. The buffer offers a working solution of Tris-Acetate-EDTA at a concentration of 50X, making it easy to dilute and use as needed. One of the standout features of this buffer is its Consistency. Users can expect Consistent results from batch to batch, which is particularly important for reproducibility in research. The buffer is pH-stable and can be stored at room temperature for convenience. Overall, the 50X TAE buffer from Cepham Life Sciences is a solid choice for researchers in need of a reliable buffer for DNA agarose gel electrophoresis.
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Pros
- Reliable and Consistent results
- pH-stable
- Convenient storage at room temperature
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Features & Benefits
- 50X TAE Buffer used for electrophoresis of nucleic acids.
- Inhibits metal dependent nucleases and protects DNA from damage.
- May be used for both genomic and large supercoiled DNA.
- Lower buffering capacity than TBE buffer.
- Available in 500mL, 1L, and 1 Gal sizes
- Not for human or animal clinical/therapeutic use
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